Coding

Part:BBa_K3452601:Design

Designed by: Hao Zhou   Group: iGEM20_CSU_CHINA   (2020-10-27)


NRAMP5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 214
    Illegal NgoMIV site found at 1168
    Illegal AgeI site found at 673
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized the codon so that this part could be better expressed in synechocystis.


Source

Oryza sativa Japonica Group genomic sequence.

References